Effect of Culture Parameters of Light Intensity and Nutrients on Germination of Alternaria Solani Conidia
Mayukh Das1, Subhadip Brahmachari2, Amitava Saha3, Surekha Kundu4
1Mayukh Das, MSc, Molecular and Applied Mycology and Plant Pathology Laboratory, Department of Botany, University of Calcutta, Kolkata (West Bengal), India.
2Subhadip Brahmachari, MSc, Molecular and Applied Mycology and Plant Pathology Laboratory, Department of Botany, University of Calcutta, Kolkata (West Bengal), India.
3Amitava Saha, MSc, Molecular and Applied Mycology and Plant Pathology Laboratory, Department of Botany, University of Calcutta, Kolkata (West Bengal), India.
4Surekha Kundu, PhD, (Corresponding author) Molecular and Applied Mycology and Plant Pathology Laboratory, Department of Botany, University of Calcutta, Kolkata (West Bengal), India.
Manuscript received on October 02, 2018. | Revised Manuscript received on October 15, 2018. | Manuscript published on October 30, 2018. | PP: 22-27 | Volume-5 Issue-10, October 2018. | Retrieval Number: J12731051018
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© The Authors. Published By: Blue Eyes Intelligence Engineering and Sciences Publication (BEIESP). This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/)
Abstract: The necrotrophic pathogen Alternaria solani fsp. Lycopersici is an important pathogen for solanaceous crops and is cultured in laboratories for various purposes. The production of spores in culture under optimum conditions is very different from that occurring in the natural environment. Therefore it is important to optimize various culture conditions to understand the effect these conditions have on the spores of the fungus. Most studies focus on sporulation rather than spore germination and growth of the germ tubes. This study investigates the effect of different culture conditions such as light intensities, darkness and presence of nutrients on spore germination and germ tube length over a time course in A. solani. Here, we observed that the germination is delayed in dark in comparison to when incubation is done in presence of light. Light appears to affect the growth of germ tubes more significantly compared to germination frequency of the spores. The implication of the findings, in the context of germination of fungi in presence of nutrients is also discussed.
Keywords: Alternaria Solani, Light Intensity, Germination Frequency, Germ Tube Length, Culture Condition.